Duddingston Kirk is a Parish Church in the Church of Scotland, located adjacent to Holyrood Park in Duddingston Village, on the east side of the City of Edinburgh. The church was built in or around 1124 by Dodin, a Norman knight, on land granted to Kelso Abbey by King David I of Scotland. As originally built, the kirk consisted of the chancel, nave and square tower. The traditional pattern of an east–west axis was adopted. The original entrance on the south wall includes a particularly fine example of Scoto-Norman stone carving, with a round-topped doorway. Following the enlargement of the parish boundaries, the Prestonfield Aisle was added in 1631. This consists of a gallery, downstairs area and burial vaults were on the north side. In 1968 the kirk’s interior was reconditioned, with the former pipe organ removed.

The entrance to the kirkyard from Duddingston village is notable for its gatehouse, built as a lookout point to deter "bodysnatchers" in the early 19th century. The Edinburgh bodysnatchers, known as "resurrectionists," stole recently buried corpses to sell to anatomists, and, as in the notorious case of Burke and Hare, sometimes also resorted to murder.